Dr.-Ing. Florian Golombek – Dynamic kinetic resolution

Dr.-Ing. Florian Golombek – Dynamic kinetic resolutions using nano-scale enzyme membrane reactors

The dynamic kinetic resolution of racemates is a powerful method for the production of enantiopure compounds. However, it is not uncommon to face incompatibilities between the cleavage and racemization reactions. Kinetic resolutions are often catalyzed by lipases via selective acylation. Unfortunately, their combination with racemases often turns out to be critical, due to the fact that such acylations normally are run in organic solvents with low water activity. In such solvents most racemases are inactive making a spatial separation of the two reactions mandatory.

Blaue Kugel mit Einschnitt auf der rechten Seite und grafischer Darstellung von roten, grünen und blauen Enzymen darin.

In this project we develop a new type of nano-scale enzyme membrane reactors for the dynamic kinetic resolutions in biphasic systems. To achieve this, polymer-based vesicles, so-called polymersomes, are used as nano-reactors with soluble enzymes encapsulated inside. To avoid disintegration by the organic solvents in the biphasic systems, the membranes of the polymersomes have to be stabilized. Additionally, the selective mass transport over the membrane has to be facilitated via  the insertion of appropriate channel proteins. After the establishment of the complete nano-reactor-based biphasic reaction system, the resulting synthesis will be thoroughly characterized and a model-supported optimization will be performed.

This project was funded by the Federal Ministry of Education and Research (Basistechnologien Nachwuchsgruppe: UniComp – Funktionelle Erweiterung polymerbasierter Reaktionskompartimente für Multienzymsynthesen, project number 031B0221).

Veröffentlichungen:

Golombek F, Haumann M, Knoll M, Fröba AP, Castiglione K (2021): Three Steps, Two Enzymes, One Pot, but a Multitude of Nanocompartments: Combined Cycles of Kinetic Resolutions and Re-racemization with Incompatible Biocatalysts. ACS Omega, 6, 43, 29192–29200.

Mertz M, Golombek F, Boye S, Moreno S, Castiglione K (2021): Fast and effective chromatographic separation of polymersomes from proteins by multimodal chromatography. J Chrom B, 1162, 122459.

Golombek F, Castiglione K (2020): Polymersomes as Nanoreactors Enabling the Application of Solvent-Sensitive Enzymes in Different Biphasic Reaction Setups. Biotechnol J, 15, 1900561.